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Trazodone hcl 100 mg sleep ) (Nominal c 0.80 mg/kg; n = 10 per group) for five consecutive nights. Each night, the rat was placed in four separate trazodone hcl 50 mg for sleep cages, identical positions, with a 6 cm×6 cm wooden barrier placed between them. The animals were acclimated at room temperature within 2 h of being placed in the cages. For this experiment, animals were given two consecutive overnight tests for safety and habituation monitored before treatment during those two tests. Each session began at 8:20 AM and ended 10:00 PM for at least two consecutive nights. The Trazodone 100mg $217.95 - $0.81 Per pill next morning at 10:00 AM, a fixed oral dose (2 mg/kg body weight) of the hcl drug was administered into either one or both eyes of each animal. After 5 days of treatment there were a single 3 × 5 cm stereotaxic (1.5T scanner, Siemens Medical Solution System) field of view (FOV) scan and a post-scan fixation period of 2 days. Data were acquired using a 3-dimensional T1-weighted sequence with the acquisition matrix set to stereotaxic space determined by the surgeon (N.V.) before scanning. Analyses were performed using the BrainVision software (www.haptex.com/bwi/faw/) (R.G. Bhattacharjee, M.N. Singh, J.D. R.N. Chaudhuri, and S.D. Kumar, unpublished data). We assessed the response to niacin determine post-treatment differences in the magnitude and pattern of its effects on locomotor activity and depression levels. Post-treatment concentrations (1–6 mg/kg) of niacin that elicited significant increases in locomotor activity were included the analysis, in each case, using a modified version of the two-way repeated measures ANOVA. Animals treated with higher doses and greater number of treatments (i.e., consecutive doses) than the lowest dose were included in the model. primary analysis of main effect treatment was the repeated-measure ANOVA for locomotion (with post-treatment minus pre-treatment numbers as factors) that was repeated for sleep, niacin, and baseline; only significant results of the two main effects were then assessed by repeated measures ANOVA with treatment as the between-subjects factor and for sleep the number of nights per treatment, to adjust for baseline differences (i.e., niacin produced sleep deficits in animals treated with increasing oral doses of niacin, with an additional sleep time advantage after the final dose that was similar to those produced by niacin at a fixed dose). In each case, we also tested for interactions between treatment and time of day from generic pharmacy uk each trial, and for group × time interactions. mean is presented in the figure. Acknowledgments The author thanks Dr. S.D. Kumar, Mr. R.G. Bhattacharjee, and Ms. J. Bajaj for their excellent technical assistance with this study as well Dr. K.K. Patel, Ms. J. Motiwala, Mr. A. Dholakia, and Dr. M. Datta for helpful comments. This work was supported by grants from the National Institutes of Health (N.V., J.K. Patel, S.D. Kumar, M.K. Sahu, T.R. Bhattacharjee, E.K. Shukla, M.C. Singh, and Dr. P.K. Mishra), NARSAD Grant No. 0112/01, the NIAAA (R.E.P.P., N.V., S.
